We study aimed to develop a mycelium-based artificial root system for improving plant growth in arid conditions. Using Pleurotus ostreatus on straw–sawdust–vermiculite substrates, 3D-printed molds (sphere, cube, cone) were tested for water absorption and strength. Straw-rich mixtures and spherical designs showed the best performance. Although limited cultivation time restricted full colonization, results indicate strong potential for porous mycelium structures to enhance water and nutrient delivery. Future work will add PGPR inoculation and plant trials to advance this eco-friendly approach for sustainable agriculture under desertification